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thruplex dna seq hv kit  (TaKaRa)


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    Structured Review

    TaKaRa thruplex dna seq hv kit
    Thruplex Dna Seq Hv Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thruplex+dna+seq+hv+kit/ThruPLEX+DNA-Seq+HV/pmc13037772-87-9-13
    Average 94 stars, based on 23 article reviews
    thruplex dna seq hv kit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: From Background to Signal: Simultaneously derived Copy Numbers from Methylation-Dependent Sequencing cell-free DNA
    Article Snippet: In short, 10ng of cfDNA was digested with LpnPI (New England Biolabs, Ipswich, MA) yielding 32 bp fragments around the fully methylated recognition site containing a CpG. .. Samples were prepped for sequencing using the ThruPLEX DNA-seq 96D kit and the ThruPLEX DNA-Seq HV kit (Rubicon Genomics, Takara Bio Europe, Saint-Germain-en-Laye, France), for CRLM and ovarian cancer samples respectively, and purified on a Pippin HT system with 3% agarose gel cassettes (Sage Science, Beverly, MA). .. Libraries were multiplexed and sequenced until a total of ∼20M reads on either an Illumina HiSeq 2500 for 50 bp single-end reads (CRLM samples) or an Illumina NEXTseq2000 (ovarian cancer) for 50 bp paired-end reads, according to the manufacturer’s instructions (Illumina, San Diego, CA).

    Article Title: Human DCM-time machine unravels cell state changes during primitive gut tube differentiation
    Article Snippet: At least 10 ng of DNA was digested by LpnPI (New England Biolabs). .. Sequencing libraries were prepped after LpnPI digestion using the ThruPLEX DNA-Seq HV kit (Takara, R400740). .. Using Pippin Prep system (Sage science) appropriate size fragments were selected.

    Article Title: Comparative Analysis of Cell-Free DNA Fragmentation Patterns in Canines with Sarcoma and Tumor-Free Canines and Humans
    Article Snippet: .. Whole-genome libraries for human samples were prepared with the ThruPLEX DNA-Seq HV kit (Takara), and sequencing was performed on the NextSeq 2000 (Illumina), as recently described (medRxiv 2024.12.13.24318579). ..

    Article Title: NeuroMabSeq: high volume acquisition, processing, and curation of hybridoma sequences and their use in generating recombinant monoclonal antibodies and scFvs for neuroscience research
    Article Snippet: .. Each of the four pools was converted into one Illumina-barcode indexed sequencing library using the ThruPLEX DNA-Seq HV kit (Takara Bio Catalog# R400740), following the manufacturer’s instructions. ..

    Article Title: Comparative analysis of cell-free DNA fragmentation patterns in canines with sarcoma and tumor-free canines and humans
    Article Snippet: .. Whole genome libraries for 165 human samples were prepared with the ThruPLEX DNA-Seq HV kit (Takara) and sequencing 166 was performed on the NextSeq 2000 (Illumina), as recently described (29). ..

    Article Title: High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research.
    Article Snippet: .. Each pool was subsequently converted into one Illumina-barcode indexed sequencing library using the ThruPLEX DNA-Seq HV kit from Takara Bio. .. To create sequence diversity for the subsequent Illumina sequencing step, the lengths of the inline indices varied between five to eight bases, staggering the readthrough through the TSO sequence shared by all amplicons.

    DNA Sequencing:

    Article Title: From Background to Signal: Simultaneously derived Copy Numbers from Methylation-Dependent Sequencing cell-free DNA
    Article Snippet: In short, 10ng of cfDNA was digested with LpnPI (New England Biolabs, Ipswich, MA) yielding 32 bp fragments around the fully methylated recognition site containing a CpG. .. Samples were prepped for sequencing using the ThruPLEX DNA-seq 96D kit and the ThruPLEX DNA-Seq HV kit (Rubicon Genomics, Takara Bio Europe, Saint-Germain-en-Laye, France), for CRLM and ovarian cancer samples respectively, and purified on a Pippin HT system with 3% agarose gel cassettes (Sage Science, Beverly, MA). .. Libraries were multiplexed and sequenced until a total of ∼20M reads on either an Illumina HiSeq 2500 for 50 bp single-end reads (CRLM samples) or an Illumina NEXTseq2000 (ovarian cancer) for 50 bp paired-end reads, according to the manufacturer’s instructions (Illumina, San Diego, CA).

    Article Title: Human DCM-time machine unravels cell state changes during primitive gut tube differentiation
    Article Snippet: At least 10 ng of DNA was digested by LpnPI (New England Biolabs). .. Sequencing libraries were prepped after LpnPI digestion using the ThruPLEX DNA-Seq HV kit (Takara, R400740). .. Using Pippin Prep system (Sage science) appropriate size fragments were selected.

    Article Title: Donor-Derived Cell-Free DNA as a Non-Invasive Biomarker for Graft Rejection in Kidney Transplant Recipients: A Prospective Study among the Indian Population
    Article Snippet: .. To perform the preamplification of cell-free DNA, we used the Takara ThruPLEX DNA-Seq HV kit (Catalog number R400740). ..

    Article Title: Comparative Analysis of Cell-Free DNA Fragmentation Patterns in Canines with Sarcoma and Tumor-Free Canines and Humans
    Article Snippet: .. Whole-genome libraries for human samples were prepared with the ThruPLEX DNA-Seq HV kit (Takara), and sequencing was performed on the NextSeq 2000 (Illumina), as recently described (medRxiv 2024.12.13.24318579). ..

    Article Title: NeuroMabSeq: high volume acquisition, processing, and curation of hybridoma sequences and their use in generating recombinant monoclonal antibodies and scFvs for neuroscience research
    Article Snippet: .. Each of the four pools was converted into one Illumina-barcode indexed sequencing library using the ThruPLEX DNA-Seq HV kit (Takara Bio Catalog# R400740), following the manufacturer’s instructions. ..

    Article Title: Comparative analysis of cell-free DNA fragmentation patterns in canines with sarcoma and tumor-free canines and humans
    Article Snippet: .. Whole genome libraries for 165 human samples were prepared with the ThruPLEX DNA-Seq HV kit (Takara) and sequencing 166 was performed on the NextSeq 2000 (Illumina), as recently described (29). ..

    Article Title: High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research.
    Article Snippet: .. Each pool was subsequently converted into one Illumina-barcode indexed sequencing library using the ThruPLEX DNA-Seq HV kit from Takara Bio. .. To create sequence diversity for the subsequent Illumina sequencing step, the lengths of the inline indices varied between five to eight bases, staggering the readthrough through the TSO sequence shared by all amplicons.

    Purification:

    Article Title: From Background to Signal: Simultaneously derived Copy Numbers from Methylation-Dependent Sequencing cell-free DNA
    Article Snippet: In short, 10ng of cfDNA was digested with LpnPI (New England Biolabs, Ipswich, MA) yielding 32 bp fragments around the fully methylated recognition site containing a CpG. .. Samples were prepped for sequencing using the ThruPLEX DNA-seq 96D kit and the ThruPLEX DNA-Seq HV kit (Rubicon Genomics, Takara Bio Europe, Saint-Germain-en-Laye, France), for CRLM and ovarian cancer samples respectively, and purified on a Pippin HT system with 3% agarose gel cassettes (Sage Science, Beverly, MA). .. Libraries were multiplexed and sequenced until a total of ∼20M reads on either an Illumina HiSeq 2500 for 50 bp single-end reads (CRLM samples) or an Illumina NEXTseq2000 (ovarian cancer) for 50 bp paired-end reads, according to the manufacturer’s instructions (Illumina, San Diego, CA).

    Agarose Gel Electrophoresis:

    Article Title: From Background to Signal: Simultaneously derived Copy Numbers from Methylation-Dependent Sequencing cell-free DNA
    Article Snippet: In short, 10ng of cfDNA was digested with LpnPI (New England Biolabs, Ipswich, MA) yielding 32 bp fragments around the fully methylated recognition site containing a CpG. .. Samples were prepped for sequencing using the ThruPLEX DNA-seq 96D kit and the ThruPLEX DNA-Seq HV kit (Rubicon Genomics, Takara Bio Europe, Saint-Germain-en-Laye, France), for CRLM and ovarian cancer samples respectively, and purified on a Pippin HT system with 3% agarose gel cassettes (Sage Science, Beverly, MA). .. Libraries were multiplexed and sequenced until a total of ∼20M reads on either an Illumina HiSeq 2500 for 50 bp single-end reads (CRLM samples) or an Illumina NEXTseq2000 (ovarian cancer) for 50 bp paired-end reads, according to the manufacturer’s instructions (Illumina, San Diego, CA).

    other:

    Article Title: High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research.
    Article Snippet: Hybridoma cells were lysed by resuspending the cell pellet in 150 μL of RNeasy Lysis Buffer (RLT) (Qiagen Catalog# 1015750) and transferred to the corresponding wells of a 96 well U bottom master block plate (Greiner Catalog# 780215).



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